Human ASAM / CLMP Protein (His Tag)

ACAM,ASAM,CSBM,CSBS

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Catalog Number	P10794-H08H
Organism Species	Human
Host	Human Cells
Synonyms	ACAM,ASAM,CSBM,CSBS
Molecular Weight	The secreted recombinant human ASAM comprises 227 amino acids and predicts a molecular mass of 25.8 kDa. It migrates as an approximately 32-35 kDa band in SDS-PAGE under reducing conditions.
predicted N	Gly 18
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain of human ASAM (NP_079045.1) precursor (Met 1-Met 233) was expressed with a C-terminal polyhistidine tag.
Bio-activity	Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line . When 4 x 10E4 cells/well are added to human ASAM coated plates (30 μg/ml, 100 μl/well), approximately > 40 % will adhere after one hour at 37 ℃.
Research Area	Neuroscience |Neurology process |Neurodegeneration and Neurodegenerative Disease |Others in Neurodegeneration and Neurodegenerative Disease
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. ASAM protein is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. In addition, its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity.
Reference	Eguchi J, et al. (2005) Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity. Biochem J. 387(Pt 2): 343-53. Sze KL, et al. (2008) Expression of CLMP, a novel tight junction protein, is mediated via the interaction of GATA with the Kruppel family proteins, KLF4 and Sp1, in mouse TM4 Sertoli cells. J Cell Physiol. 214(2): 334-44. Sze KL, et al. (2008) Post-transcriptional regulation of CLMP mRNA is controlled by tristetraprolin in response to TNFalpha via c-Jun N-terminal kinase signalling. Biochem J. 410(3): 575-83.