Human Alkaline Phosphatase / ALPI Protein (Fc Tag)
IAP
- 100ug (NPP1893) Please inquiry
| Catalog Number | P13225-H02H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | IAP |
| Molecular Weight | The secreted recombinant human ALPI/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 725 amino acids and has a predicted molecular mass of 79.5 kDa. The apparent molecular mass of rhALPI /Fc monomer is approximately 90-95 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Val 20 |
| SDS-PAGE |  |
| Purity | > 83 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503),without the pro peptide, was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 10,000 pmoles/min/μg. |
| Research Area | Cancer |Signal transduction |Phosphatase & Regulator |Phosphatase Regulator |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |