Human Alkaline Phosphatase / ALPI Protein (His Tag)

IAP

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Catalog Number	P13225-H08H
Organism Species	Human
Host	Human Cells
Synonyms	IAP
Molecular Weight	The secreted recombinant human ALPI consists of 495 amino acids and has a predicted molecular mass of 54 kDa. The apparent molecular mass of rhALPI is approximately 66 kDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N	Val 20
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503),without the pro peptide, was fused with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 1,000 pmoles/min/μg.
Research Area	Cancer |Signal transduction |Phosphatase & Regulator |Phosphatase Regulator
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Nakano T, et al. (2009) Role of lysophosphatidylcholine in brush-border intestinal alkaline phosphatase release and restoration. Am J Physiol Gastrointest Liver Physiol. 297(1): G207-14. Henthorn PS, et al. (1987) Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase. Proc Natl Acad Sci. 84(5): 1234-8. Oetting WS, et al. (2003) Oculocutaneous albinism type 1: the last 100 years. Pigment Cell Res. 16(3): 307-11.