Human B3GNT2 Protein (Fc Tag)
B3GN-T2,B3GNT,B3GNT-2,B3GNT1,BETA3GNT,BGnT-2,BGNT2
- 100ug (NPP1920) Please inquiry
Catalog Number | P11416-H01H |
---|---|
Organism Species | Human |
Host | Human Cells |
Synonyms | B3GN-T2,B3GNT,B3GNT-2,B3GNT1,BETA3GNT,BGnT-2,BGNT2 |
Molecular Weight | The recombinant human B3GNT2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 629 amino acids and has a predicted molecular mass of 71.2 kDa. The apparent molecular mass of the protein is approximately 112-120 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
predicted N | Glu |
SDS-PAGE | |
Purity | > 90 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the human B3GNT2 (Q9NY97-1) (Lys29-Cys397) was expressed,with the fused Fc region of human IgG1 at the N-terminus. |
Bio-activity | |
Research Area | |
Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | B3GNT2 belongs to the beta-1,3-N-acetylglucosaminyltransferase family. It is a type II transmembrane protein which prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. B3GNT2 catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increases poly-N-acetyllactosamine chains by activating intrinsic B3GNT2. |
Reference |