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Human B3GNT2 Protein (Fc Tag)

B3GN-T2,B3GNT,B3GNT-2,B3GNT1,BETA3GNT,BGnT-2,BGNT2

Catalog Number P11416-H01H
Organism Species Human
Host Human Cells
Synonyms B3GN-T2,B3GNT,B3GNT-2,B3GNT1,BETA3GNT,BGnT-2,BGNT2
Molecular Weight The recombinant human B3GNT2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 629 amino acids and has a predicted molecular mass of 71.2 kDa. The apparent molecular mass of the protein is approximately 112-120 kDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N Glu
SDS-PAGE
Purity > 90 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human B3GNT2 (Q9NY97-1) (Lys29-Cys397) was expressed,with the fused Fc region of human IgG1 at the N-terminus.
Bio-activity
Research Area
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background B3GNT2 belongs to the beta-1,3-N-acetylglucosaminyltransferase family. It is a type II transmembrane protein which prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. B3GNT2 catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increases poly-N-acetyllactosamine chains by activating intrinsic B3GNT2.
Reference
  • Australo-An, et al. (2010) Genome-wide association study of ankylosing spondylitis identifies non-MHC susceptibility loci. Nat Genet. 42(2):123-7.
  • Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.
  • Seko A, et al. (2008) Activation of beta1,3-N-acetylglucosaminyltransferase-2 (beta3Gn-T2) by beta3Gn-T8. Possible involvement of beta3Gn-T8 in increasing poly-N-acetyllactosamine chains in differentiated HL-60 cells. J Biol Chem. 283(48):33094-100.