Human B7-DC / PD-L2 / CD273 / PDCD1LG2 Protein (Fc Tag)

B7-DC,B7DC,bA574F11.2,Btdc,CD273,PD-L2,PDCD1L2,PDL2

100ug (NPP1072) Please inquiry

Catalog Number	P10292-H02H
Organism Species	Human
Host	Human Cells
Synonyms	B7-DC,B7DC,bA574F11.2,Btdc,CD273,PD-L2,PDCD1L2,PDL2
Molecular Weight	The recombinant human PDCD1LG2 consists 438 amino acids and predicts a molecular mass of 49.3 kDa.
predicted N	Leu 20
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE.
Protein Construction	A DNA sequence encoding the human PDCD1LG2 (NP_079515.2) (Met1-Pro219) was expressed with the Fc region of human IgG1 at the C-terminus.
Bio-activity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized human PD1-His (P10377-H08H) at 10 μg/mL (100 μL/well) can bind human PDCD1LG2-Fc. The EC₅₀ of PDCD1LG2-Fc is 0.05-0.12 μg/mL.
Research Area	Immunology \|Innate Immunity \|Monocytes/Macrophages \|Co-stimulatory Molecules
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.
Reference	Latchman Y, et al. (2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8. Carreno BM, et al. (2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30. Radhakrishnan S, et al. (2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.