Human B7-DC / PD-L2 / CD273 Protein (His & Fc Tag)
B7-DC,B7DC,bA574F11.2,Btdc,CD273,PD-L2,PDCD1L2,PDL2
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| Catalog Number | P10292-H03H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | B7-DC,B7DC,bA574F11.2,Btdc,CD273,PD-L2,PDCD1L2,PDL2 |
| Molecular Weight | The recombinant human PD-L2/Fc is a disulfide-linked homodimeric protein after proteolytic removal of the signal peptide. The reduced monomer consists of 447 amino acids and predicts a molecular mass of 50.4 kDa. As a result of glycosylation, the apparent molecular mass of rh PD-L2/Fc monomer is approximately 70-80 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Leu 20 |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Pro 219) of human PD-L2 (NP_079515.2) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA . Immobilized human PD1 at 2 μg/ml (100 μl/well) can bind human PD-L2 with a linear ranger of 0.0064-0.16 μg/ml . |
| Research Area | Immunology |Innate Immunity |Monocytes/Macrophages |Co-stimulatory Molecules |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1. |
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