Human CADM3 / NECL1 / IGSF4B Protein (His Tag)
BIgR,IGSF4B,Necl-1,NECL1,synCAM3,TSLL1
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| Catalog Number | P11214-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | BIgR,IGSF4B,Necl-1,NECL1,synCAM3,TSLL1 |
| Molecular Weight | The recombinant human CADM3 consists of 317 amino acids and predictes a molecular mass of 35.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CADM3 is approximately 39-41 kDa due to glycosylation. |
| predicted N | Asn 25 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human CADM3 isoform 2 (short isoform) (NP_001120645.1) extracellular domain (Met 1-His 330) was expressed, with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. Human CADM3 immobilized (0.8 μg/ml, 100 μl/well) will mediate >30% C6 cell adhesion. |
| Research Area | Cancer |Oncoprotein & suppressor & biomarker |Tumor biomarker |Tumor Suppressor |Other in Tumor suppressor |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Cell Adhesion Molecules (CAMs) are proteins located on the cell surface involved with the binding with other cells or with the extracellular matrix (ECM) in the process called cell adhesion. These proteins are typically transmembrane receptors and are composed of three domains: an intracellular domain that interacts with the cytoskeleton, a transmembrane domain, and an extracellular domain that interacts either with other CAMs of the same kind (homophilic binding) or with other CAMs or the extracellular matrix (heterophilic binding). Cell adhesion molecule 3, also known as Immunoglobulin superfamily member 4B, CADM3, and NECL1, is a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule which has Ca(2+)-independent homo- or heterophilic cell-cell adhesion activity and plays an important role in the formation of synapses, axon bundles and myelinated axons. Isoform 1 of CADM3 is expressed mainly in adult and fetal brain. Isoform 2 of CADM3 is highly expressed in adult brain and weakly expressed in placenta. In brain, Isoform 2 is highly expressed in cerebellum. CADM3 is involved in the cell-cell adhesion. It has both calcium-independent homophilic cell-cell adhesion activity and calcium-independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. The interaction with EPB41L1 may regulate structure or function of cell-cell junctions. CADM3 may act as a tumor suppressor in glioma and loss of it in glioma may be caused by histone deacetylation. |
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