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Human CAMK1G / CLICK III / CaMKIgamma Protein (His & GST Tag)

CLICK3,CLICKIII,dJ272L16.1,VWS1

Catalog Number P13148-H20B
Organism Species Human
Host Baculovirus-Insect Cells
Synonyms CLICK3,CLICKIII,dJ272L16.1,VWS1
Molecular Weight The recombinant human CAMK1G/GST chimera consists of 713 amino acids and has a calculated molecular mass of 81 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
predicted N Met
SDS-PAGE
Purity > 85 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human CAMK1G isoform 1 (Q96NX5-1) (Met 1-Met 476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity No Kinase Activity
Research Area Neuroscience |Neurotransmission |Calcium Signaling |Calmodulin Pathway
Formulation Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 0.3mM DTT
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background Calmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Concentration of cytosolic calcium functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calcium binding proteins (calmodulin/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases referred to as CaM-kinases (CaMKs), such as CaMKI, CaMKII, CaMKIV and CaMKK. Calmodulin-dependent protein kinase CL3/CaMKIγ is a memberane-anchored CaMK belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKIγ membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKIγ critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKIγ specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKIγ may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons. 
Reference
  • Davare MA, et al. (2009) Transient receptor potential canonical 5 channels activate Ca2+ / calmodulin kinase Igamma to promote axon formation in hippocampal neurons. J Neurosci. 29 (31): 9794-808.
  • Takemoto-Kimura S, et al. (2007) Regulation of dendritogenesis via a lipid-raft-associated Ca2+ / calmodulin - dependent protein kinase CLICK-III / CaMKIgamma. Neuron. 54 (5): 755-70.
  • Harrill JA, et al. (2010) Splice variant specific increase in Ca2+ / calmodulin-dependent protein kinase 1-gamma mRNA expression in response to acute pyrethroid exposure. J Biochem Mol Toxicol. 24 (3): 174-86.