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Human CAMKV Protein (His & GST Tag)

1G5,VACAMKL

Catalog Number P12243-H20B
Organism Species Human
Host Baculovirus-Insect Cells
Synonyms 1G5,VACAMKL
Molecular Weight The recombinant human CAMKV/GST chimera consists of 738 amino acids and has a calculated molecular mass of 82.2 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
predicted N Met
SDS-PAGE
Purity > 80 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human CAMKV (NP_076951.2) (Met 1-Ser 501) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity No Kinase Activity
Research Area Cancer |Signal transduction |Signaling Pathway |Calcium Signaling |Calmodulin Pathway
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background CaM kinase-like vesicle-associated protein, also known as CAMKV, is a peripheral membrane protein and Cytoplasmic vesicle membrane protein which belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. CAMKV contains one protein kinase domain. It is predominantly observed in association with the plasma membrane of soma and in neurites, both axons and dendrites. CAMKV may be associated with vesicular structures. It does not appear to have detectable kinase activity.
Reference
  • Hunter T, et al.,1988, Science. 241 (4861): 42-51.
  • Wiemann S., et al., 2001, Genome Res. 11:422-435.
  • G. Manning, et al., 2002, Science 6. 298:1912-1934.
  • Manning G, et al.,2002, Science. 298 (5600): 1912-34.
  • Ota T., et al., 2004, Nat. Genet. 36:40-45.