Human CD112 / Nectin-2 / PVRL2 Protein
CD112,HVEB,Nectin-2,PRR2,PVRR2
- 100ug (NPP1112) Please inquiry
| Catalog Number | P10005-HCCH |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD112,HVEB,Nectin-2,PRR2,PVRR2 |
| Molecular Weight | The recombinant human CD112 comprises 335 amino acids and has a calculated molecular mass of 36.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions. |
| predicted N | Gln 32 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE and SEC-HPLC Analysis. |
| Protein Construction | The mature form of recombinant human CD112 (NP_002847.1) extracellular domain (Met 1-Leu 360) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human CD112 (P 10005-HCCH) at 10 μg/ml (100 μl/well) can bind human DNAM1-Fch (P 10565-H03H) with a linear range of 1.25-10 μg/ml. |
| Research Area | Developmental Biology |Embryogenesis |Germ Layer Formation |Ectoderm Marker |
| Formulation | Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy. |
| Reference |