Human CD122 / IL-2RB Protein (Fc Tag)
CD122,IL15RB,P70-75
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| Catalog Number | P10696-H02H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD122,IL15RB,P70-75 |
| Molecular Weight | The recombinant human IL2 Rβ/Fc is a disulfide-linked homodimeric protein generated after removal of the signal peptide. The reduced monomer consists of 451 amino acids and has a predicted molecular mass of 51.6 kDa. As a result of glycosylation, the apparent molecular mass of rh IL2RB/Fc monomer is approximately 60-65 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Ala 27 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Asp 239) of human IL2 Rβ (NP_000869.1) precursor was expressed with the C-terminal fused Fc region of human IgG1. |
| Bio-activity | 1. Measured by its ability to bind biotinylated recombinant rat IL2 in a functional ELISA. 2. Using the Octet RED System, the affinity constant (Kd) of Human IL2RB-Fc (P10696-H02H) bound to Human IL2 was 0.8nM. |
| Research Area | Immunology |Cytokines & Growth Factors |Cytokine & Receptor |Interleukin & Receptor |Other Interleukin & Receptor |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development. |
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