Human CD146 / MCAM Protein (His Tag)
CD146,MUC18
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| Catalog Number | P10115-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD146,MUC18 |
| Molecular Weight | The recombinant CD146 comprises 547 amino acids and predicts a molecular mass of 61.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Val 24 |
| SDS-PAGE |  |
| Purity | > 98 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain of human CD146 precursor (NP_006491.2) (Met 1-Gly 559) was expressed with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line . When 3 x 10E4 cells/well are added to human CD146 coated plates (0.8 μg/ml, 100 μl/well), approximately >50 % will adhere after one hour at 37 ℃. |
| Research Area | Developmental Biology |Embryogenesis |Germ Layer Formation |Mesoderm Marker |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas. |
| Reference |