Human CD27 / TNFRSF7 Protein (Fc Tag)
S152,S152. LPFS2,S152.LPFS2,T14,TNFRSF7,Tp55
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| Catalog Number | P10039-H31H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | S152,S152. LPFS2,S152.LPFS2,T14,TNFRSF7,Tp55 |
| Molecular Weight | The recombinant human CD27/rFc is a disulfide-linked homodimer. The reduced monomer comprises 396 amino acids and has a predicted molecular mass of 44.4 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Ala 20 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human CD27 (P26842) (Met1-Ile192) was expressed, fused with the Fc region of rabbit IgG at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human CD27-his (P10039-H31H) at 10 μg/mL (100 μl/well) can bind biotinylated human CD70-Fc (P10780-H01H), The EC50 of biotinylated human CD70-Fc (P10780-H01H) is 95-150 ng/mL. |
| Research Area | Cancer |Signal transduction |Other Related Intracellular Topics |Regulation of Apoptosis by TNF Superfamily Members |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. |
| Reference |