Human CD32a / FCGR2A Protein (167 Arg, His & AVI Tag), Biotinylated
CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2
- 100ug (NPP1153) Please inquiry
| Catalog Number | P10374-H27H-B |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2 |
| Molecular Weight | The recombinant human CD32a consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 kDa. It migrates as an approximately 32 kDa protein due to glycosylation by SDS-PAGE under reducing conditions. |
| predicted N | Gln 34 |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Met 210) of human CD32a (AAA35827.1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. It is identical to FCGR2A131H/R in the reference. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD32a(P10374-H27H-B). The EC50 of Human CD32a(P10374-H27H-B) is 1.6 - 3.8 μg/ml. 2. Labeling ratio of biotin to protein: 0.3 |
| Research Area | Immunology |Adaptive Immunity |B Cell |B Cell CD Antigen |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |