Call Now

Human CD32a / FCGR2A Protein (167 Arg) (His & AVI Tag)

CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2

Catalog Number P10374-H27H
Organism Species Human
Host Human Cells
Synonyms CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2
Molecular Weight The recombinant human CD32a consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 kDa. It migrates as an approximately 32 kDa protein due to glycosylation by SDS-PAGE under reducing conditions.
predicted N Gln 34
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the extracellular domain (Met 1-Met 210) of human CD32a (AAA35827.1) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus. It is identical to FCGR2A131H/R in the reference.
Bio-activity Immobilized human CD32a-AVI-His (R131) (P10374-H27H) at 10 μg/ml (100 μl/well) can bind biotinylated Human IgG1, The EC50 of biotinylated Human IgG1 is 0.15-0.35 μg/ml.
Research Area Immunology |Adaptive Immunity |B Cell |B Cell CD Antigen
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference