Human CD32a / FCGR2A Protein (167 His, His Tag)
CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2
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| Catalog Number | P10374-H08C1 |
|---|---|
| Organism Species | Human |
| Host | CHO Cells |
| Synonyms | CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2 |
| Molecular Weight | The secreted recombinant human CD32a consists of 196 amino acids and predicts a molecular mass of 22 KDa. The apparent molecular mass of the protein is approximately 29-32 KDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Gln 34 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding (Met 1-Ile 218) of human CD32a (NP_001129691.1) was expressed with a C-terminal polyhistidine tag. It is identical to FCGR2A131H/R in the reference. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human CD32a-His (R131H) (CHO) (P10374-H08C1) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.09-0.21 μg/ml. |
| Research Area | Immunology |Adaptive Immunity |B Cell |B Cell CD Antigen |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |