Human CD32b / FCGR2B Protein (His & AVI Tag), Biotinylated
CD32B,Fc gamma RIIB,FCGR2
- 100ug (NPP1161) Please inquiry
| Catalog Number | P10259-H27H-B |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD32B,Fc gamma RIIB,FCGR2 |
| Molecular Weight | The recombinant mature human CD32b consists of 214 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 35 kDa protein due to glycosylation. |
| predicted N | Ala 46 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Ala 46-Ile 224) of mature human CD32b (NP_001002274.1) protein was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD32b(P10259-H27H-B). The EC50 of Human CD32b(P10259-H27H-B) is 1.6 - 3.7 μg/ml. 2. Labeling ratio of biotin to protein: 0.5 |
| Research Area | Immunology |Innate Immunity |Granulocyte Markers |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
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