Human CD32b / FCGR2B Protein (His Tag)
CD32B,Fc gamma RIIB,FCGR2
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| Catalog Number | P10259-H08C |
|---|---|
| Organism Species | Human |
| Host | CHO Cells |
| Synonyms | CD32B,Fc gamma RIIB,FCGR2 |
| Molecular Weight | The secreted recombinant human CD32b consists of 183 amino acids and predicts a molecular mass of 20.8 KDa. The apparent molecular mass of the protein is approximately 29 KDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Ala 46 |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding (Ala46-Pro217) of human CD32b (P31994-1) was expressed,with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human sCD32b-His (CHO) (P10259-H08C) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.18-0.42 μg/ml. |
| Research Area | Immunology |Innate Immunity |Granulocyte Markers |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |