Human CD32b / FCGR2B Protein (His Tag)

CD32B,Fc gamma RIIB,FCGR2

Catalog Number	P10259-H08H
Organism Species	Human
Host	Human Cells
Synonyms	CD32B,Fc gamma RIIB,FCGR2
Molecular Weight	The recombinant mature human CD32b consists of 183 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 20.8 kDa. By SDS-PAGE under reducing conditions, it migrates as an approximately 25-30 kDa protein due to glycosylation.
predicted N	Ala 46
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain (Ala 46-Pro 217) of mature human CD32b (NP_001002274.1) protein was expressed with a N-terminal signal peptide and a C-terminal polyhistidine tag.
Bio-activity	Measured by its binding ability in a functional ELISA . Immobilized recombinant human CD32b at 10 μg/ml (100 μl/well) can bind human IgG2 with a linear range of 0.16-6.4 μg/ml.
Research Area	Immunology \|Innate Immunity \|Granulocyte Markers
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference