Human CD34 Protein (His Tag)

RP11-328D5.2, CD34

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Catalog Number	P10103-H08H
Organism Species	Human
Host	Human Cells
Synonyms	RP11-328D5.2, CD34
Molecular Weight	The secreted recombinant human CD34 consists of 270 amino acids after removal of the signal peptide and has a predicted molecular mass of 29 kDa. In SDS-PAGE under reducing conditions, rh CD34 migrates with the apparent molecular mass betwwen 40 and 50 kDa due to different glycosylation.
predicted N	Ser 32
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain of human CD34 precursor (NP_001020280.1) (Met 1-Thr 290) was expressed with a polyhistidine tag at the C-terminus.
Bio-activity	1. Measured by its ability to bind human CD62L in a functional ELISA . 2. Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 4 x 10E4 cells/well are added to human CD34 coated plates (0.8 μg/ml, 100 μl/well), approximately >40 % will adhere after one hour at 37 ℃ .
Research Area	Developmental Biology |Embryogenesis |Germ Layer Formation |Mesoderm
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.
Reference	Hogan CJ, et al. (2002) Differential long-term and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice. Proc Nat Acad Sci USA. 99 (1): 413-8. Nielsen JS,et al. (2009) CD34 is a key regulator of hematopoietic stem cell trafficking to bone marrow and mast cell progenitor trafficking in the periphery. Microcirculation. 16(6): 487-96. Mastrandrea F,et al. (2009) CD34+ hemopoietic precursor and stem cells traffic in peripheral blood of celiac patients is significantly increased but not directly related to epithelial damage severity. Eur Ann Allergy Clin Immunol. 40(3): 90-103. Pasquet S,et al. (2009) Long-term benefit of intracardiac delivery of autologous granulocyte-colony-stimulating factor-mobilized blood CD34+ cells containing cardiac progenitors on regional heart structure and function after myocardial infarct. Cytotherapy. 11(8): 1002-15.