Human CD3D & CD3E Heterodimer Protein
CD3-DELTA,IMD19,T3D
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| Catalog Number | PCT038-H2508H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD3-DELTA,IMD19,T3D |
| Molecular Weight | The recombinant heterodimer of human CD3D&CD3E comprises 272 (126+146) amino acids and has a calculated molecular mass of 31.2 (14.4+16.8)KDa. As a result of glycosylation, the apparent molecular mass of human CD3D&CD3E heterodimer is approximately 20, 22 and 26 KDa in SDS-PAGE under reducing conditions. |
| predicted N | Phe 22 & Asp 23 |
| SDS-PAGE |  |
| Purity | (43.8+13.1+41.9) % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein (PCT038-H2508H) at 10 μg/ml (100 μl/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 7.0-18.0 ng/ml. |
| Research Area | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |