Human CD3D & CD3E Heterodimer Protein
CD3-DELTA,IMD19,T3D
- 100ug (NPP1990) Please inquiry
| Catalog Number | PCT026-H0323H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD3-DELTA,IMD19,T3D |
| Molecular Weight | The recombinant heterodimer of human CD3D/CD3E comprises 670 (333+354) amino acids and has a calculated molecular mass of 77.5 (37.5+40） kDa. As a result of glycosylation, the apparent molecular mass of human CD3D/CD3E heterodimer is approximately 45-50 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Phe 22 & Asp 23 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of human CD3D (P04234) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (P07766) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein (PCT026-H0323H) at 10 μg/ml (100 μl/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 0.07-0.17 μg/ml. |
| Research Area | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |