Human CD3E & CD3G Heterodimer Protein
CD3E & CD3G Heterodimer,IMD18,T3E,TCRE
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| Catalog Number | PCT041-H0305H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD3E & CD3G Heterodimer,IMD18,T3E,TCRE |
| Molecular Weight | The recombinant heterodimer of human CD3E/CD3G comprises 683 (355+328) amino acids and has a calculated molecular mass of 77.2 (40.1+37.1) KDa. As a result of glycosylation, the apparent molecular mass of human CD3E/CD3G heterodimer is approximately 42-47 KDa in SDS-PAGE under reducing conditions. |
| predicted N | Asp 23 & Gln 23 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (NP_000724.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Ser116) of human CD3G (NP_000064.1) was fused the Fc region of mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified. |
| Bio-activity | Measured by its ability to bind OKT3-mIgG2a in a functional ELISA. |
| Research Area | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |