Human CD6 / Cluster of Differentiation 6 Protein (Fc Tag)
TP120
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| Catalog Number | P17051-H02H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | TP120 |
| Molecular Weight | The recombinant human CD6 consists of 619 amino acids and predicts a molecular mass of 67.3 kDa. |
| predicted N | His 18 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE. |
| Protein Construction | A DNA sequence encoding the human CD6 (NP_006716.3) (Met1-Glu398) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8×10E4 cells/well are added to CD6-Fc coated plates (10 μg/mL, 100 μL/well), approximately >70% will adhere after 60 minutes at 37℃. |
| Research Area | Immunology |Inflammation / Inflammatory Mediator |Cells Involved in Inflammation |Monocyte/Macrophage |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains three SRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse. |
| Reference |