Human CD64 / FCGR1A Protein (His & AVI Tag), Biotinylated
CD64,Fc gamma RI
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| Catalog Number | P10256-H27H-B |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD64,Fc gamma RI |
| Molecular Weight | The secreted recombinant human CD64 consists of 307 amino acids and has a predicted molecular mass of 34.4 kDa. |
| predicted N | Gln 16 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain of human CD64 (NP_000557.1) (Met 1-Thr 287) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant CD64-AVI-His (P10256-H27H-B) at 10 μg/ml (100 μl/well) can human IgG1 with a linear range of 1.56-50 ng/ml. 3. Labeling ratio of biotin to protein: 1.09. |
| Research Area | Immunology |Cluster of Differentiation (CD) |Monocyte/Macrophage CD Antigen |Macrophage Marker CD Antigen |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses. |
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