Human CD64 / FCGR1A Protein (His Tag)
CD64,Fc gamma RI
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| Catalog Number | P10256-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD64,Fc gamma RI |
| Molecular Weight | The recombinant human CD64A consists of 284 amino acids and has a predicted molecular mass of 32 kDa. |
| predicted N | Gln 16 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain of human CD64A (NP_000557.1) (Met 1-Pro 288) was expressed, fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA . 1. Immobilized CD64 at 10 μg/ml can bind human IgG1 with a linear range of 0.39-25 ng/ml. 2. Scatchard analysis showed the affinity constant (Kd) of CD64 bound to Human IgG1 was 0.059 nM . |
| Research Area | Immunology |Cluster of Differentiation (CD) |Monocyte/Macrophage CD Antigen |Macrophage Marker CD Antigen |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses. |
| Reference |