Human CD7 Protein (His Tag)

GP40,LEU-9,Tp40,TP41

100ug (NPP1993) Please inquiry

Catalog Number	P11028-H08H
Organism Species	Human
Host	Human Cells
Synonyms	GP40,LEU-9,Tp40,TP41
Molecular Weight	The secreted recombinant human CD7 consists of 166 amino acids and predictes a molecular mass of 18 kDa. In SDS-PAGE under reducing conditions, it migrates with apparent molecular mass of 30-35 kDa due to glycosylation.
predicted N	Ala 26
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human CD7 (NP_006128.1) extracellular domain (Met 1-Pro 180) was expressed, with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized human CD7-His at 10 μg/ml (100 μl /well) can bind biotinylated SECTM1-His(P12269-H08H), The EC₅₀ of biotinylated SECTM1-His(P12269-H08H) is 11-26ng/ml
Research Area	Immunology \|Signal Transduction \|Transcription Factors and Regulators \|Immunoglobulin-Like Domain Transcription Factors
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD7 is a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell / B-cell interaction during early lymphoid development.
Reference	Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5. Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.