Human CDC42 / G25K Protein (GST Tag)

CDC42Hs,G25K

• 100ug (NPP1996) Please inquiry

Catalog Number	P10062-H09E
Organism Species	Human
Host	E. coli
Synonyms	CDC42Hs,G25K
Molecular Weight	The recombinant human CDC42/GST chimera consists of 423 amino acids and has a predicted molecular mass of 48.1 kDa. It migrates as an approxiamtely 44 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 85 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human CDC42 isoform 2 (P60953-2) (Met 1-Cys 188) was fused with the GST tag at the N-terminus.
Bio-activity
Research Area	Signaling |Signal Transduction |Other Signal Transduction Molecules
Formulation	Lyophilized from sterile 20mM Tris, 0.15M NaCl, 0.5mM GSH, pH 8.0 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	et al. Shinjo K, et al. (1990) Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42. Proc Natl Acad Sci. 87(24):9853-7. Munemitsu S, et al. (1990) Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42. Mol Cell Biol. 10(11):5977-82. Walker S J, et al. (2000) Activation of phospholipase D1 by Cdc42 requires the Rho insert region. J Biol Chem. 275(21):15665-8. Low B C, et al. (1999) Tyrosine phosphorylation of the Bcl-2-associated protein BNIP-2 by fibroblast growth factor receptor-1 prevents its binding to Cdc42GAP and Cdc42. J Biol Chem. 274(46): 33123-30. Zhang B, et al. (1998) Interaction of Rac1 with GTPase-activating proteins and putative effectors. A comparison with Cdc42 and RhoA. J Biol Chem. 273(15):8776-82.