Human CDK2AP2 Protein (His Tag)
DOC-1R,p14
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| Catalog Number | P12552-H08E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | DOC-1R,p14 |
| Molecular Weight | The recombinant human CDK2AP2 consists of 136 amino acids and has a calculated molecular mass of 14.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human CDK2AP2 is approximately 18 and 12 kDa due to different glycosylation. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 78 %(the upper band)+17 %(the lower band) as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human CDK2AP2 (O75956) (Met 1-Thr 126) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | |
| Research Area | Immunology |Signal Transduction |Akt Pathway |Phospholipases, Small GTPases, and Other Molecules in the Akt Pathway |
| Formulation | Lyophilized from sterile PBS, 15% glycerol, pH 7.5 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | CDK2AP2 belongs to the CDK2AP family. Members of this family of proteins are cell-growth suppressors, associating with and influencing the biological activities of important cell cycle regulators in the S phase including monomeric non-phosphorylated cyclin-dependent kinase 2 (CDK2) and DNA polymerase alpha/primase. CDK2AP2 contains 5 distinct gt-ag introns. Transcription produces 7 different mRNAs, 6 alternatively spliced variants and 1 unspliced form. There are 2 non overlapping alternative last exons and 4 validated alternative polyadenylation sites. The mRNAs appear to differ splicing versus retention of 3 introns. CDK2AP2 plays a role in regulating self-renewal of mouse embryonic stem cells (mESC) under permissive conditions, and cell survival during differentiation of the mESC into terminally differentiated cell types. |
| Reference |