Human CFL1 / N-cofilin Protein (His Tag)
CFL1, CFL
- 100ug (NPP2007) Please inquiry
| Catalog Number | P14544-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | CFL1, CFL |
| Molecular Weight | The recombinant human CFL1 consists of 181 amino acids and predicts a molecular mass of 20.3 KDa. It migrates as an approximately 21 KDa band in SDS-PAGE under reducing conditions. |
| predicted N | His |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human CFL1 (P23528) (Met1-Leu166) was expressed with a polyhistidine tag at the N-terminus. |
| Bio-activity | |
| Research Area | Immunology |Signal Transduction |Cytoskeleton / ECM |Cytoskeletal Proteins |Microfilaments |Actin etc | |
| Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | CFL1, also known as n-cofilin, is a member of the ADF/Cofilin family. This family comprises three genes: CFL1, CFL2 and DSTN (destrin). ADF/Cofilin family members bind G-actin monomers and depolymerize actin filaments through two mechanisms: severing and increasing the off-rate for actin monomers from the pointed end. Cofilin also binds with other proteins such as myosin, tropomyosin, α-actinin, gelsolin and scruin. These proteins compete with cofilin for actin binding. Сofilin also plays a role in innate immune response. CFL1 contains 1 ADF-H domain and is widely distributed in various tissues. It is important for normal progress through mitosis and normal cytokinesis. |
| Reference |