Human CHST15 / GALNAC4S-6ST / BRAG Protein (His Tag)
BRAG,GALNAC4S-6ST
- 100ug (NPP3886) Please inquiry
| Catalog Number | P11033-H07H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | BRAG,GALNAC4S-6ST |
| Molecular Weight | The recombinant human BRAG consists of 479 amino acids and predictes a molecular mass of 56 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh BRAG is approximately 70-80 kDa due to glycosylation. |
| predicted N | His |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human BRAG (NP_056976.2) extracellular domain (Ser 99-Thr 561) was expressed, with a polyhistidine tag at the N-terminus. |
| Bio-activity | |
| Research Area | Immunology |Signal Transduction |Metabolism |Types of disease |Metabolism in Cancer |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Carbohydrate sulfotransferase 15, also known as N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase, GalNAc4S-6ST, B-cell RAG-associated gene protein, CHST15 and BRAG, is a single-pass type I I membrane protein which belongs to the sulfotransferase 1 family. CHST15 / BRAG is expressed in B-cell-enriched tissues but not in fetal or adult thymus. It is expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. It is not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. CHST15 / BRAG is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. CHST15 / BRAG may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and / or regulation of B-cell-specific RAG expression. |
| Reference |