Human CNPY4 Protein (His Tag)

PRAT4B

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Catalog Number	P13355-H08H
Organism Species	Human
Host	Human Cells
Synonyms	PRAT4B
Molecular Weight	The recombinant human CNPY4 consists of 238 amino acids and predicts a molecular mass of 27.4 KDa. It migrates as an approximately 32 KDa band in SDS-PAGE under reducing conditions.
predicted N	Gly 22
SDS-PAGE
Purity	> 85 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human CNPY4 (Q8N129) (Met1-Leu248) was expressed with a polyhistidine tag at the C-terminus.
Bio-activity
Research Area	Cancer |Signal transduction |Metabolism |Lipid metabolism |Cholesterol Metabolism
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	CNPY4 belongs to the canopy family. CNPY4 interacts with toll-like receptor 4 (TLR4) and plays a role in the regulation of the cell surface expression of TLR4. Toll-like receptors (TLRs) recognize microbial products and induce immune responses. Lipopolysaccharide is recognized by the receptor complex consisting of TLR4 and MD-2. As CNPY4, PRAT4B also regulates cell surface expression of TLR4. PRAT4B has a signal peptide followed by a mature peptide. It is associated with the hypoglycosylated, immature form of TLR4 but not with MD-2 or TLR2.
Reference	Stelzl U, et al. (2005) A human protein-protein interaction network: a resource for annotating the proteome. Cell. 122(6):957-68. Hocking JC, et al. (2010) Distinct roles for Robo2 in the regulation of axon and dendrite growth by retinal ganglion cells. Mech Dev. 127(1-2):36-48. Hart BE, et al. (2012) Cell surface trafficking of TLR1 is differentially regulated by the chaperones PRAT4A and PRAT4B. J Biol Chem. 287(20):16550-62.