Human CPNE1 / Copine I / CPN1 Protein

CPNE1, CPN1

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Catalog Number	P14283-HNCB
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	CPNE1, CPN1
Molecular Weight	The recombinant human CPNE1 consists of 539 amino acids and predicts a molecular mass of 59.2 KDa. It migrates as an approximately 69 KDa band in SDS-PAGE under reducing conditions.
predicted N	Gly
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human CPNE1(Q99829) (Met1-Ala537) was expressed and purified.
Bio-activity
Research Area	Signaling |Signal Transduction |Signaling Pathway |Calcium Signaling |Others in Calcium Signaling
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.0. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Copine I, also known as CPN1, is a member of the copine family. Copine I is a calcium-dependent membrane-binding protein which has a wide tissue distribution. Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. Copine I contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus, while it does not contain a predicted signal sequence or transmembrane domains. Copine I may function in membrane trafficking.
Reference	Cowland JB, et al. (2003) Tissue expression of copines and isolation of copines I and III from the cytosol of human neutrophils. J Leukoc Biol. 74(3):379-88. Tomsig JL, et al. (2003) Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif. J Biol Chem. 278 (12):10048-54. Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.