Human CRADD / RAIDD Protein (His Tag)
MRT34,RAIDD
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| Catalog Number | P12900-H08E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | MRT34,RAIDD |
| Molecular Weight | The recombinant human CRADD consisting of 209 amino acids and has a calculated molecular mass of 24.1 kDa. It migrates as an approximately 26 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human CRADD (P78560) (Met 1-Glu 199) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | |
| Research Area | Cancer |Signal transduction |Signaling Pathway |Representative pathway |Apoptosis Signaling pathway |Apoptosis Adaptor Proteins | |
| Formulation | Lyophilized from sterile PBS, 20% glycerol, pH 8.0 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Death domain-containing protein CRADD, also known as Caspase and RIP adapter with death domain, RIP-associated protein with a death domain, CRADD and RAIDD, is a protein which is constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney. CRADD / RAIDD contains one CARD domain and one death domain. CRADD / RAIDD contains a death domain involved in the binding of RIP protein. The CARD domain mediates the interaction with caspase-2. FADD / MORT1 is a death domain (DD)-containing adaptor / signaling molecule that interacts with the intracellular DD of FAS / APO-I ( CD95 ) and tumor necrosis factor receptor 1 and the prodomain of caspase-8 ( Mch5 / MACH / FLICE). CRADD / RAIDD has a dual-domain structure similar to that of FADD. CRADD / RAIDD has an NH2-terminal caspase homology domain that interacts with caspase-2 and a COOH-terminal DD that interacts with RIP. CRADD / RAIDD could play a role in regulating apoptosis in mammalian cells. CRADD / RAIDD is a apoptotic adaptor molecule specific for caspase-2 and FASL / TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD / RAIDD recruits caspase-2 to the TNFR-1 signalling complex. |
| Reference |