Human CRIP2 Protein (His & GST Tag)
CRIP,CRP2,ESP1
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| Catalog Number | P14552-H20B |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | CRIP,CRP2,ESP1 |
| Molecular Weight | The recombinant human CRIP2/GST chimera consists of 445 amino acids and has a calculated molecular mass of 50.3 kDa. The recombinant protein migrates as an approximately 49 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human CRIP2 (P52943) (Met1-Pro208) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
| Bio-activity | |
| Research Area | Epigenetics |Transcription |Domain Families |LIM |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | CRIP2 is a putative transcription factor. It has a widespread tissue expression and is highly expressed in heart. CRIP2 contains two LIM zinc-binding domains. CRIP2 may participate in the differentiation of smooth muscle tissue. It also plays an important role in esophageal squamous cell carcinoma (ESCC) tumorigenesis. CRIP2 acts as a transcription repressor of the NF-κB-mediated proangiogenic cytokine expression and thus functionally inhibits tumor formation and angiogenesis. It interacts with the NF-κB/p65 to inhibit its DNA-binding ability to the promoter regions of the major proangiogenesis cytokines critical for tumor progression, including IL6, IL8, and VEGF. In conclusion, we provide compelling evidence that CRIP2 acts as a transcription repressor of the NF-κB-mediated proangiogenic cytokine expression and thus functionally inhibits tumor formation and angiogenesis. |
| Reference |