Human CSNK1A1 / CKI-alpha / CK1 Protein (GST Tag)
CK1,CK1a,CKIa,HEL-S-77p,HLCDGP1,PRO2975
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| Catalog Number | P10668-H09B |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | CK1,CK1a,CKIa,HEL-S-77p,HLCDGP1,PRO2975 |
| Molecular Weight | The recombinant human CSNK1A1/GST chimera consists of 561 amino acids and predicts a molecular mass of 65.2 kDa. It migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the full length of human CSNK1A1 isoform 1 (P48729-1) (Met 1-Phe 337) was expressed with the GST tag at the N-terminus. |
| Bio-activity | The specific activity was determined to be 30 nmol/min/mg using casein as substrate. |
| Research Area | Immunology |Signal Transduction |Signaling Pathway |Representative pathway |Hedgehog Signaling Pathway |
| Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, 2mM GSH, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Casein kinase I isoform alpha, also known as CKI-alpha, CK1 and CSNK1A1, is a cytoplasm protein which belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. High expression of CSNK2A1, or concomitantly high expression of CSNK2A1, are independent prognostic factors of poor survival in NSCLC patients. CSNK2A1 are useful prognosis markers in non-small cell lung cancer (NSCLC) patients after complete resection, independent of lymph node metastasis status. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. It phosphorylates CTNNB1 at 'Ser-45'. CSNK1A1 may play a role in segregating chromosomes during mitosis. |
| Reference |