Human CTLA4 / CD152 Protein (His Tag)

ALPS5,CD,CD152,CELIAC3,CTLA-4,GRD4,GSE,IDDM12

100ug (NPP1208) Please inquiry

Catalog Number	P11159-H08H
Organism Species	Human
Host	Human Cells
Synonyms	ALPS5,CD,CD152,CELIAC3,CTLA-4,GRD4,GSE,IDDM12
Molecular Weight	The recombinant human CTLA4 consists of 137 amino acids and has a predicted molecular mass of 15 kDa.
predicted N	Lys 36
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) was fused with the a polyhistidine tag at the C-terminus.
Bio-activity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized human CTLA4 (cat: 11159-H08H) at 10 μg/mL (100 μl/well) can bind human B7-1/Fc (cat: 10698-H03H), The EC₅₀ of human B7-1/Fc (cat: 10698-H03H is 14 ng/mL. 3. Immobilized human CTLA4 (cat: 11159-H08H) at 10 μg/mL (100 μl/well) can bind human B7-2/Fch (cat: 10699-H03H), The EC₅₀ of human B7-2/Fch (cat: 10699-H03H) is 53 ng/mL.
Research Area	Immunology \|Adaptive Immunity \|Costimulation & Costimulatory Molecule \|B7/CD28 Family
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).
Reference	Slavik JM, et al. (1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24. Holmberg D, et al. (2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33. Chin LT, et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.