Human Carbonic Anhydrase VIII / CA8 Protein (His Tag)
CA-VIII,CALS,CAMRQ3,CARP,MGC120502,MGC99509
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| Catalog Number | P10469-H08E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | CA-VIII,CALS,CAMRQ3,CARP,MGC120502,MGC99509 |
| Molecular Weight | The recombinant human CA8 comprises 296 amino acids and has a predicted molecular mass of 33.8 kDa. It migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met 1 |
| SDS-PAGE |  |
| Purity | > 94 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human CA8 (NP_004047.3) (Met 1-Gln 290) was expressed, with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its esterase activity . The specific activity is >100 pmoles/min/μg. |
| Research Area | Developmental Biology |Metabolism |Pathways and Processes |Metabolism processes |Apoptosis |Intracellular | |
| Formulation | Lyophilized from sterile PBS, 15% glycerol, pH 7.5 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer. |
| Reference |