Human Complexin-2 / CPLX2 Protein (His Tag)

921-L,CPX-2,CPX2,Hfb1

• 100ug (NPP3760) Please inquiry

Catalog Number	P11927-H07E
Organism Species	Human
Host	E. coli
Synonyms	921-L,CPX-2,CPX2,Hfb1
Molecular Weight	The recombinant human CPLX2 consists of 144 amino acids and has a calculated molecular mass of 16.8 kDa. It migrates as an 21 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human CPLX2 (Q6PUV4-1) (Asp 2-Lys 134) was expressed, with a polyhistide tag at the N-terminus.
Bio-activity
Research Area	Cancer |Signal transduction |Other Related Intracellular Topics |Other Apoptosis Molecules
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Complexin-2 (CPLX2), a member of the complexin/synaphin family, is a soluble pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals. Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Complexins are unable to bind to monomeric SNARE proteins but bind with high affinity to ternary SNARE complexes and with lower affinity to target SNARE complexes. Complexin 1 (CX1) and complexin 2 (CX2) are presynaptic proteins that modulate neurotransmitter release and are used as markers of inhibitory and excitatory synapses, respectively. CPLX2 is localized in pre-synaptic terminals in mature brain. The G71-P89 region of CPLX2 is essential and sufficient for preferential axonal distribution. CPLX2 participates in the Ca(2+)-sensitive regulatory pathway for zymogen granule exocytosis. Complexin-2 is a key player in normal neurological function, and its downregulation could lead to changes in neurotransmitter release sufficient to cause significant behavioural abnormalities such as depression. It is involved in synaptogenesis and the modulation of neurotransmitter release.
Reference	Lee HJ, et al. (2005) Association study of polymorphisms in synaptic vesicle-associated genes, SYN2 and CPLX2, with schizophrenia. Behav Brain Funct. 1: 15. Salimi K, et al. (2008) Regulation of complexin 1 and complexin 2 in the developing human prefrontal cortex. Synapse. 62(4): 273-82. Kataoka M, et al. (2009) Identification of a minimal segment of complexin II essential for preferential distribution in axons. J Neurochem. 108(5): 1109-15. Glynn D, et al. (2010) Clorgyline-mediated reversal of neurological deficits in a Complexin 2 knockout mouse. Hum Mol Genet. 19(17): 3402-12. Falkowski MA, et al. (2010) Complexin 2 modulates vesicle-associated membrane protein (VAMP) 2-regulated zymogen granule exocytosis in pancreatic acini. J Biol Chem. 285(46): 35558-66.