Human Cystatin 7 / CST7 Protein (His Tag)
CMAP
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| Catalog Number | P10167-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CMAP |
| Molecular Weight | Cystatin F exists as a disulfide-linked homodimer. The human cystatin F monomer consists of 137 amino acids and and has a predicted molecular mass of 16.1 kDa. As a result of glycosylation, the recombinant Cystatin F migrates as an approximately 44 kDa band in SDS-PAGE under non-reduced conditions corresponding to the dimmer. |
| predicted N | Gly 20 |
| SDS-PAGE |  |
| Purity | > 92 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human Cystatin F precursor (NP_003641.3) (Met 1-His 145) was expressed，with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog # ES008). The IC50 value is <6 nM. |
| Research Area | Immunology |Adaptive Immunity |T Cell |Non-CD of T cell |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis. |
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