Human Cystatin 7 / CST7 Protein
CMAP
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Catalog Number | P10167-HCCH |
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Organism Species | Human |
Host | Human Cells |
Synonyms | CMAP |
Molecular Weight | The recombinant human Cystatin F consists of 132 amino acids and predicts a molecular mass of 15.2 kDa. As a result of glycosylation, the apparent molecular mass of rhCystatin F is approximately 25 kDa in SDS-PAGE. |
predicted N | Gly 20 |
SDS-PAGE | |
Purity | > 95 % as determined by SDS-PAGE |
Protein Construction | The mature form of the human Cystatin F (NP_003641.3) (Met 1-His 145) with five amino acids (DDDDK) at the C-terminus was expressed and purified. |
Bio-activity | |
Research Area | Immunology |Adaptive Immunity |T Cell |Non-CD of T cell |
Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis. |
Reference |