Human DCUN1D1 / SCCRO Protein (His Tag)
DCNL1,DCUN1L1,RP42,SCCRO,SCRO,Tes3
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| Catalog Number | P15059-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | DCNL1,DCUN1L1,RP42,SCCRO,SCRO,Tes3 |
| Molecular Weight | The recombinant human DCUN1D1 consists of 274 amino acids and predicts a molecular mass of 32 KDa. It migrates as an approximately 28-32 KDa band in SDS-PAGE under reducing conditions. |
| predicted N | His |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human DCUN1D1 (NP_065691.2) (Met1-Val259) was expressed with a polyhistidine tag at the N-terminus. |
| Bio-activity | |
| Research Area | Cancer |Oncoprotein & suppressor & biomarker |Oncoprotein |other in oncoprotein |
| Formulation | Lyophilized from sterile PBS, 10% glycerol, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | DCUN1D1, also known as SCCRO, is part of an E3 ubiquitin ligase complex for neddylation. DCUN1D1 functions to recruit charged E2 and is involved in the release of inhibitory effects of CAND1 on cullin-RING ligase E3 complex assembly and activity. DCUN1D1 binds to the components of the neddylation pathway (Cullin-ROC1, Ubc12, and CAND1) and augments but is not required for cullin neddylation in reactions using purified recombinant proteins. DCUN1D1 also recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation in purified protein assays. DCUN1D1 also acts as am ncogene facilitating malignant transformation and carcinogenic progression. |
| Reference |