Human DDR2 Kinase / CD167b Protein (Fc Tag)

MIG20a,NTRKR3,TKT,TYRO10

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Catalog Number	P10209-H02H
Organism Species	Human
Host	Human Cells
Synonyms	MIG20a,NTRKR3,TKT,TYRO10
Molecular Weight	The recombinant human DDR2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 616 amino acids and has a calculated molecular mass of 69.4 kDa. Due to glycosylation, rhDDR2/Fc monomer migrates as an approximately 87 kDa protein in SDS-PAGE under reducing conditions.
predicted N	Lys 22
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Arg 399) of human DDR2 precursor (NP_001014796.1) was expressed with the fused Fc region of human IgG1 at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized Rat tail Collagen I at 10 μg/ml can bind recombinant human DDR2-Fc Chimera with a linear range of 2.5-80 ng/ml. Scatchard analysis showed the affinity constant (Kd) of recombinant human DDR2-Fc Chimera bound to rat tail collagen I was 6.8 nM .
Research Area	Signaling |Signal Transduction |Receptor Tyrosine Kinases (RTKs)
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.
Reference	et al. Olaso E, et al. (2001) DDR2 receptor promotes MMP-2-mediated proliferation and invasion by hepatic stellate cells. J Clin Invest. 108(9): 1369-78. Zhang W, et al. (2006) Expression of discoidin domain receptor 2 (DDR2) extracellular domain in pichia pastoris and functional analysis in synovial fibroblasts and NIT3T3 cells. Mol Cell Biochem. 290(1-2): 43-53. Leitinger B, et al. (2006) The discoidin domain receptor DDR2 is a receptor for type X collagen. Matrix Biol. 25(6): 355-64. Leitinger B, et al. (2004) The D2 period of collagen II contains a specific binding site for the human discoidin domain receptor, DDR2. J Mol Biol. 344(4): 993-1003.