Human DEP1 / PTPRJ Protein (aa 997-1337, His Tag)
CD148,DEP1,HPTPeta,R-PTP-ETA,SCC1
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| Catalog Number | P13165-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | CD148,DEP1,HPTPeta,R-PTP-ETA,SCC1 |
| Molecular Weight | The recombinant human PTPRJ (aa 997-1337) consists of 352 amino acids and has a calculated molecular mass of 41KDa. It migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human PTPRJ (Q12913) cytoplasmic domain (Arg 997-Ala 1337) was expressed, with a polyhistide tag at the N-terminus. |
| Bio-activity | |
| Research Area | Cancer |Signal transduction |Phosphatase & Regulator |Phosphatase Regulator |
| Formulation | Lyophilized from sterile PBS, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | DEP1 / PTPRJ (Receptor-type tyrosine-protein phosphatase eta) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. DEP1 / PTPRJ possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. DEP1 / PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. In stable MCF-7 cell lines lines, induction of DEP-1 expression inhibited breast cancer cell growth by 5-10-fold. These data describe PTPs expressed and regulated in breast cancer cell lines during differentiation and identify one PTP, DEP-1, that inhibits the growth of breast cancer cells in vitro. |
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