Human DKKL1 / Soggy-1 / SGY-1 Protein (Fc Tag)
CT34,SGY,SGY-1,SGY1
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| Catalog Number | P10840-H01H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CT34,SGY,SGY-1,SGY1 |
| Molecular Weight | The recombinant human DKKL1/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 472 amino acids and has a predicted molecular mass of 52.4 kDa. rhDKKL1/Fc monomer migrates as an approximately 60 kDa protein in SDS-PAGE under reducing conditions. |
| predicted N | Glu |
| SDS-PAGE |  |
| Purity | > 70 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the secreted form of human DKKL1 (Q9UK85) (Ala 31-Leu 242) was expressed with the fused Fc region of human IgG1 at the N-terminus. |
| Bio-activity | Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 5-15 μg/ml. |
| Research Area | Immunology |Signal Transduction |Other Related Intracellular Topics |Cellular Senescence and Pathways in Aging |Apoptosis |Nuclear | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Dickkopf-like 1 (DKKL1) or soggy 1, is a glycoprotein unique to mammals that is expressed primarily in developing spermatocytes and localized in the acrosome of mature sperm. It is also expressed in the trophectoderm / placental lineage. This glycoprotein is secreted by postmeiotic male germ cells. DKKL1 is a member of the Dickkopf (DKK) family, a group of proteins that are characterized as secreted antagonists of Wnt signal transduction proteins. In mammals, embryos lacking DKKL1 protein developed into viable, fertile adults. DKKL1, either directly or indirectly, facilitates the ability of sperm to penetrate the zona pellucid. DKKL1 is related to the sperm apoptotic procession. Molecular analyses identified the Fas death ligand (FasL) as a target for DkkL1 pro-apoptotic activity in adult mice. DKKL1 is considered as a negative regulator of adult testic homeostasis and identifies a novel, DKKL1 / FasL- dependent, regulation that specifically controls the number of postpubertal spermatocytes. |
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