Human DNASE1 / Deoxyribonuclease I / DNL1 Protein (His Tag)
DNL1,DRNI
- 100ug (NPP2082) Please inquiry
| Catalog Number | P13801-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | DNL1,DRNI |
| Molecular Weight | The recombinant human DNASE1 consists of 271 amino acids and predicts a molecular mass of 30.7 KDa. It migrates as an approximately 37 KDa band in SDS-PAGE under reducing conditions. |
| predicted N | Leu 23 |
| SDS-PAGE |  |
| Purity | > 85 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human DNASE1 (P24855) (Met1-Lys282) was expressed with a polyhistidine tag at the C-terminus. |
| Bio-activity | |
| Research Area | Cell Biology |Apoptosis |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | DNase1, also known as deoxyribonuclease I and DNL1, is a member of the DNase family. DNaseI is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. DNaseI binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. Mutations in DNase1 gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. DNase1 is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. |
| Reference |