Human Defensin / Beta-defensin 3 / DEFB103 Protein (His Tag)

BD-3,DEFB-3,DEFB103,DEFB3,hBD-3,HBD3,HBP-3,HBP3

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Catalog Number	P10849-H07E
Organism Species	Human
Host	E. coli
Synonyms	BD-3,DEFB-3,DEFB103,DEFB3,hBD-3,HBD3,HBP-3,HBP3
Molecular Weight	The recombinant human DEFB103A consists of 61 amino acids and predicts a molecular mass of 7.3 KDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 99 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human DEFB103A (P81534) (Gly 23-Lys 67) was expressed, with a polyhistidine tag at the N-terminus.
Bio-activity
Research Area	Cardiovascular |Atherosclerosis |Vascular Inflammation |Inflammatory mediators
Formulation	Lyophilized from sterile 50mM Tris, 0.3% Ttiton X-100, 0.3% SKL, pH 8.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Beta-defensin 3 is a member of the defensin family. Defensin family is comprised by microbicidal and cytotoxic peptides made by neutrophils. Members of the beta-defensin 3 family are highly similar in protein sequence. Beta-defensin 3 shows antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Beta-defensin 3 is abundantly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. It is also expressed in salivary gland, bone marrow, colon, stomach, polyp and larynx. However, in small intestine, it cannot be detected. Defensin has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. Beta-defensin 3 kills multiresistant S.aureus and vancomycin-resistent E.faecium. It has no significant hemolytic activity.
Reference	Garca JR, et al. (2002) Identification of a novel, multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction. Cell Tissue Res. 306(2):257-64. Dunsche A, et al. (2002) The novel human beta-defensin-3 is widely expressed in oral tissues. Eur J Oral Sci. 110(2):121-4. Abiko Y, et al. (2004) Upregulated expression of human beta defensin-1 and -3 mRNA during differentiation of keratinocyte immortalized cell lines, HaCaT and PHK16-0b. J Dermatol Sci. 31(3): 225-8.