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Human ECD / Ecdysoneless homolog Protein (His & GST Tag)

ECD,GCR2,HSGT1

Catalog Number P14311-H20B
Organism Species Human
Host Baculovirus-Insect Cells
Synonyms ECD,GCR2,HSGT1
Molecular Weight The recombinant human ECD/GST chimera consists of 881 amino acids and has a calculated molecular mass of 100.6 kDa. The recombinant protein migrates as an approximately 88-108 kDa band in SDS-PAGE under reducing conditions.
predicted N Met
SDS-PAGE
Purity > 90 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human ECD (O95905-1) (Met1-Thr621) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity
Research Area Immunology |Signal Transduction |Cellular Senescence and Pathways in Aging |Apoptosis |Apoptosis Receptors |Associated Proteins |
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background ECD, also known as ecdysoneless homolog, belongs to the SGT1 family. It is highly expressed in muscle and heart. ECD is a novel promoter of mammalian cell cycle progression. This function is related to its ability to remove the repressive effects of Rb-family tumor suppressors on E2F transcription factors. It is a novel tumor-promoting factor that is differentially expressed in pancreatic cancer and potentially regulates glucose metabolism within cancer cells. ECD may also be a transcriptional activator required for the expression of glycolytic genes.
Reference
  • Badzek S. et al., 2011, Wien Klin Wochenschr. 123 (23-24): 726-31.
  • Zhao X. et al., 2012, Breast Cancer Res Treat. 134 (1): 171-80.
  • Dey P. et al., 2012, Clin Cancer Res. 18 (22): 6188-98.