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Human ECH1 Protein (His Tag)

ECH1

Catalog Number P14566-H07E
Organism Species Human
Host E. coli
Synonyms ECH1
Molecular Weight The recombinant human ECH1 consists of 310 amino acids and predicts a molecular mass of 34 KDa. It migrates as an approximately 34 KDa band in SDS-PAGE under reducing conditions.
predicted N His
SDS-PAGE
Purity > 90 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the mature form of human ECH1 (Q13011) (Thr34-Leu328) was expressed with a polyhistide tag at the N-terminus.
Bio-activity
Research Area Signaling |Signal Transduction |Metabolism |Pathways and Processes |Metabolic signaling pathways |Lipid and lipoprotein metabolism |
Formulation Lyophilized from sterile 20mM Tris, 0.1M NaCl, 10% Glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
Reference
  • Kovalyov LI, et al. (2006) Polymorphism of delta3,5-delta2,4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue. Biochemistry Mosc. 71(4): 448-53.
  • Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 127(3):635-48.
  • FitzPatrick DR, et al. (1995) Isolation and characterization of rat and human cDNAs encoding a novel putative peroxisomal enoyl-CoA hydratase. Genomics. 27(3):457-66.