Human ECH1 Protein (His Tag)
ECH1
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Catalog Number | P14566-H07E |
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Organism Species | Human |
Host | E. coli |
Synonyms | ECH1 |
Molecular Weight | The recombinant human ECH1 consists of 310 amino acids and predicts a molecular mass of 34 KDa. It migrates as an approximately 34 KDa band in SDS-PAGE under reducing conditions. |
predicted N | His |
SDS-PAGE | |
Purity | > 90 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the mature form of human ECH1 (Q13011) (Thr34-Leu328) was expressed with a polyhistide tag at the N-terminus. |
Bio-activity | |
Research Area | Signaling |Signal Transduction |Metabolism |Pathways and Processes |Metabolic signaling pathways |Lipid and lipoprotein metabolism | |
Formulation | Lyophilized from sterile 20mM Tris, 0.1M NaCl, 10% Glycerol, pH 8.0. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. |
Reference |