Human EG-VEGF / prokineticin-1 Protein (His Tag)

EGVEGF,PK1,PRK1

100ug (NPP1580) Please inquiry

Catalog Number	P10183-H08B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	EGVEGF,PK1,PRK1
Molecular Weight	The secreted recombinant human EG-VEGF consists of 96 amino acids and predicts a molecular mass of 11 KDa. The apparent molecular mass of the protein is approximately 15 Kda in SDS-PAGE under reducing conditions due to glycosylation.
predicted N	Ala 20
SDS-PAGE
Purity	> 89 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human EG-VEGF (Met 1-Phe105) (P58294) was expressed, with a C-terminal polyhistidine tag.
Bio-activity
Research Area	Cancer \|Oncoprotein & suppressor & biomarker \|Oncoprotein \|Growth Factor & Receptor \|Other Growth Factors & Receptors \|Other Growth Factors \|
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 0.02% Tween-80, 10% gly, 1mM DTT 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	EG-VEGF, also known as prokineticin-1, is a member of the AVIT (prokineticin) family. Prokineticins are secreted proteins that can promote angiogenesis and induce strong gastrointestinal smooth muscle contraction. EG-VEGF can be detected in the steroidogenic glands, ovary, testis, adrenal and placenta. EG-VEGF has little or no effect on a variety of other endothelial and non-endothelial cell types. It induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. EG-VEGF may play a role in placentation. It may also function in normal and pathological testis angiogenesis. It positively regulates PTGS2 expression and prostaglandin synthesis.
Reference	Masuda Y, et al. (2002) Isolation and identification of EG-VEGF/prokineticins as cognate ligands for two orphan G-protein-coupled receptors. Biochem. Biophys. Res. Commun. 293 (1): 396-402. Pasquali D. et al. (2006) The endocrine-gland-derived vascular endothelial growth factor (EG-VEGF)/prokineticin 1 and 2 and receptor expression in human prostate: Up-regulation of EG-VEGF/prokineticin 1 with malignancy. Endocrinology. 147 (9): 4245-51. Ngan ES, et al. (2008) Prokineticin-1 (Prok-1) works coordinately with glial cell line-derived neurotrophic factor (GDNF) to mediate proliferation and differentiation of enteric neural crest cells. Biochim Biophys Acta. 1783 (3): 467-78. Ngan ES, et al. (2007) Prokineticin-1 modulates proliferation and differentiation of enteric neural crest cells. Biochim Biophys Acta. 1773 (4): 536-45.